IN VITRO ANTICOAGULANT EFFECTS OF DIFFERENT TYPES OF MUMIE ASSESSED BY THE LEE–WHITE METHOD
Abstract
Mumie is a natural mineral–organic substance widely used in traditional medicine for various therapeutic purposes. However, its effects on blood coagulation may vary depending on geographical origin and chemical composition. To compare the in vitro effects of different types of mumie on whole-blood coagulation using the Lee–White method. Whole-blood coagulation time was assessed in vitro according to the Lee–White method. Samples of Afghan, Indian, Chinese, and Transbaikal mumie were tested at concentrations ranging from 1 to 10 mg/mL. Control samples were analyzed without mumie. Coagulation time was recorded in minutes and compared across different mumie types and concentrations. Afghan, Indian, and Transbaikal mumie demonstrated pronounced anticoagulant activity, characterized by a dose-dependent prolongation of blood coagulation time. At concentrations of 2–5 mg/mL, coagulation time increased up to 30 minutes, while Afghan mumie at 10 mg/mL prolonged coagulation time to 90 minutes. In contrast, Chinese mumie exhibited minimal effects on coagulation, with only slight increases in clotting time across tested concentrations.
Keywords
Mumie, blood coagulation, anticoagulant activity, Lee–White method, traditional medicine, in vitro study.
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